World Health Organization : Year 1985-86, Lab 86.1: Standardized Romanowsky Staining of Blood and Bone Marrow Films Prepared on Behalf of the World Health Organization

By S. M. Lewis, Dr.

Book Id: WPLBN0000051105
Format Type: PDF eBook:
File Size: 0.3 MB
Reproduction Date: 2005

Title:	World Health Organization : Year 1985-86, Lab 86.1: Standardized Romanowsky Staining of Blood and Bone Marrow Films Prepared on Behalf of the World Health Organization
Author:	S. M. Lewis, Dr.
Volume:
Language:	English
Subject:	Health., Public health, Wellness programs
Collections:	Medical Library Collection, World Health Collection
Historic Publication Date:
Publisher:	World Health Organization


Citation APA MLA Chicago M. Lewi, B. S. (n.d.). World Health Organization : Year 1985-86, Lab 86.1. Retrieved from http://gutenberg.cc/

Description
Medical Reference Publication

Excerpt
1. INTRODUCTION Stained blood and bone marrow films have an essential role in the diagnosis of blood disorders. The Romanowsky stains are universally employed for the staining of these films. The remarkable property of the Romanowsky dyes to make subtle distinctions in shades of staining between different constituents of cells, depends on two components, namely, azure B and eosin Y. Different combinations of these stains have been used and the combinations have been given different names according to their originators, e.g, Hay-Griinwald, Giemsa, Leishman, and Wright. These differences cause variable staining. In addition, variation in the purity of the dyes used in any stain may cause batch-to-batch variation.

Table of Contents
CONTENTS Page 1. INTRODUCTION. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 2. METHOD OF STAINING BLOOD AND BOXE MARROW . . . . . . . . . . . . . . . . . . . . . 2 2.1 Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 2.2 Chemicals .......................................................2 2.3 Stock solut. io.n.s .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .; .. .. .. .. .. .. .. .. .. .. .. .. .. .. 3 2.4 Staining jar procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2.5 Dropper procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2.6 Rapid stain for emergency use . . . . . . . . . . . . . . . . . . . . . . . . 4 2.7 Malaria parasites . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 2.8 Coverglass . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 3. ROMANOWSKY STAIN EFFECT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 4. QUALITY ASSURANCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 4.1 Qualitycontrol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 4.2 Storage of azure B - eosin Y stock solution . . . . . . . . . . . . . . . . . 6 4.3 Film preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 4.4 Clean slides . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 4.5 Drying of stained films . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 APPENDIX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7