World Health Organization : Year 1997 ; World Health Organization, Laboratory, No. 97.2: World Health Organization Calibration and Control of Basic Blood Cell Counters

By J. M. England

Book Id: WPLBN0000145592
Format Type: PDF eBook:
File Size: 0.5 MB
Reproduction Date: 2005

Title:	World Health Organization : Year 1997 ; World Health Organization, Laboratory, No. 97.2: World Health Organization Calibration and Control of Basic Blood Cell Counters
Author:	J. M. England
Volume:
Language:	English
Subject:	Health., Public health, Wellness programs
Collections:	Medical Library Collection, World Health Collection
Historic Publication Date:
Publisher:	World Health Organization


Citation APA MLA Chicago M. Englan, B. J. (n.d.). World Health Organization : Year 1997 ; World Health Organization, Laboratory, No. 97.2. Retrieved from http://gutenberg.cc/

Description
Medical Reference Publication

Excerpt
INTRODUCTION The commonly used blood cell counters function on the principle of aperture impedance as originally described in 1956 by W.H. Coulter. There are now many types of electronic counters which use this process; thcy are of varying degrees of complexity and cost: (a) fully automated systems which measure all the components of a full blood count, including thc differential leucocyte count; (b) semi-automated systems which are preset to count the cells and also to measure Packed Cell Volume (PCV) and Mean Cell Volume (MCV) on whole blood or on prediluted samples; (c) simple single-channel instruments which require the operator to set the instrumcnt for counting red blood cells and leucocytes in separate procedures in prediluted solutions of a blood sample. This type of instrument was described in a previous WHO document.' it is relatively cheap and is generally adequate for the needs of a small laboratory. Only this category of instrument will be considered here.

Table of Contents
CONTENTS WIlOlLABl97.2 page 2 Page 1 . INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2 . RED BLOOD CELL COUNT W C ) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2.1 Blood sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2.2 Diluent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2.3 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2.4 Method for establishing threshold . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 23 Calibrator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2.6 Qualitycontrol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2.7 Pat*~ ent>s d ata . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 3 . TOTAL WHITE BLOOD CELL (JJEUCOCYTE) COUNT (WCC) . . . . . . . . . . . . . . . . . . . . . 7 3.1 Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 3.3 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 3.3 Quality control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 4 . APPENDIX . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 4.1 Red blood cell count (RCC) by visual (counting chamber) method . . . . . . . . . . . . . . . . . . . . . . . . . 9 4.2 Total white blood cell count (WCC)b y visual (chamber counting) method . . . . . . . . . . . . . . . . . . 10 4.3 Blood cell counter faults . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 Reference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 Figures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 I I - .... -. .........