Health Organisation, Malaria Comission; World Health Organization : Organisation Mondiale de la Sante; Serie de Monographies, Issue No. 80. 917: Isolation of Plasmodium Falciparijm Merozoites from in Vitro Cultures by Passage Through a Concanavalin A-Sepharose Column

By Xavier Billiault

Book Id: WPLBN0000090174
Format Type: PDF eBook:
File Size: 0.8 MB
Reproduction Date: 2005

Title:	Health Organisation, Malaria Comission; World Health Organization : Organisation Mondiale de la Sante; Serie de Monographies, Issue No. 80. 917: Isolation of Plasmodium Falciparijm Merozoites from in Vitro Cultures by Passage Through a Concanavalin A-Sepharose Column
Author:	Xavier Billiault
Volume:
Language:	English
Subject:	Health., Public health, Wellness programs
Collections:	Medical Library Collection, World Health Collection
Historic Publication Date:
Publisher:	World Health Organization


Citation APA MLA Chicago Billiault, B. X. (n.d.). Health Organisation, Malaria Comission; World Health Organization : Organisation Mondiale de la Sante; Serie de Monographies, Issue No. 80. 917. Retrieved from http://gutenberg.cc/

Description
Medical Reference Publication

Excerpt
This work received financial assistance from the UNDP/WORLD BANK@ Special Programme for Research and Training in Tropical Diseases. The issue of this document does not constitute Ce document ne constitue pas une publication. formal publication. It should not be reviewed, II ne doit faire I'objet d'aucun compte rendu ou abstracted or quoted without the agreement of resum6 ni d'aucune citation sans I'autorisation de the World Health Organization. Authors alone I'Organisation Mondiale de la Sante. Les opinions are responsible for views expressed in signed exprimhes dans les articles signes n'engagent articles. gue leurs auteurs.

Table of Contents
CONTENTS ENGLISH ONLY (avec resum6 en f r a n ~ a i s ) . -Page 1. I n t r o d u c t i o n . . . . . . . . . . . . . . . , . . . . . . . . . . . . . . . . . . . 2 2. M a t e r i a l a n d m e t h o d s . . . . . . . . . . . . . . . . . . . . . . . . . . . . , . . 2 2.1 Experimental set-up . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 2.2 Human serum and erythrocytes . . . . . , , . . . . . . . . . . . . , , . . . 2 2.3 Plasmodium falciparum s t r a i n . . . . . . . . . . . . . . . . . . . . . . . . 3 2.4 Parasitaemia . . . . . . . . . . . . . . . . . . . . . , . . . . . . , . . . 3 2.5 Counting. . . . . . , . . . . . . . . . , . . . , . . . . . . , , . . . , . 3 2.6 Injection of a suspension of erythrocytes . . . . . . . . . . . . . . . . . 3 2.7 Concanavalin A-Sepharose gel . . . . . . . .'. . . . . . . . . . . . . . . . 3 3. Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 3.1 Elution of erythrocytes . . . . . , . , . . . . . , . . . . , , , . . . . . 3 3.2 Elution ofmerozoites . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3.3 Viability of the merozoites . . . . . . . . . . . , . . . . . , , . . . . . 4 3.4 Purity of the merozoite suspensions . . . . . . . . . . . . . . . . . . . . 4 4. D i s c u s s i o n . . . . . . . . . . . . . . . . . . . . . . . . . . . . , . , . . . , . 4 5. C o n c l u s i o n . . . . . , . . . . . . . . . . . . . . . . . . . . . . . . . . , . . . 5 6. Sumary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Acknowledgements . . . . . , , , . . . , . . . . . . . , . . . . . . . . . , . . . . . 6 References . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . 6 Figures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7