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Sampling and Characterization of Bioaerosols

By Jensen, Paul A.

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Book Id: WPLBN0000121102
Format Type: PDF eBook:
File Size: 0.1 MB
Reproduction Date: 2005

Title: Sampling and Characterization of Bioaerosols  
Author: Jensen, Paul A.
Volume:
Language: English
Subject: Health., Medical research, Medical reports
Collections: Medical Library Collection
Historic
Publication Date:
Publisher: Centers for Disease Control and Prevention

Citation

APA MLA Chicago

A. Jense, B. P. (n.d.). Sampling and Characterization of Bioaerosols. Retrieved from http://gutenberg.cc/


Excerpt
INTRODUCTION: A. General. Bioaerosol monitoring is a rapidly emerging area of industrial hygiene. Bioaerosol monitoring includes the measurement of viable (culturable and nonculturable) and nonviable microorganisms in both indoor (e.g., industrial, office or residential) and outdoor (e.g., agricultural and general air quality) environments. In general, indoor bioaerosol sampling need not be performed if visible growth is observed.

Table of Contents
CONTENTS: PAGE 1. INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 a. General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 b. Indoor and Outdoor Bioaerosol Sampling . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 c. Viable and Nonviable Bioaerosol Sampling . . . . . . . . . . . . . . . . . . . . . . . . . . 84 2. PRINCIPLES OF OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 a. General Principles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86 b. Impaction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 c. Filtration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 d. Impingement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88 e. Characteristics of Several Bioaerosol Samplers . . . . . . . . . . . . . . . . . . . . . . . 89 3. SAMPLING CONSIDERATIONS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 a. Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 b. Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93 c. Flow Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94 d. Culture Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94 (1) Fungi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94 (2) Bacteria . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95 (3) Thermophilic Actinomycetes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95 (4) Additional Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95 e. Blanks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96 (1) Laboratory Media Blanks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96 (2) Field Blanks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96 4. PREPARATION, IDENTIFICATION, AND ENUMERATION PROCEDURES FOR CULTURABLE BIOAEROSOLS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96 a. Sample Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96 b. Enumeration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 (1) Total Concentration (Colony Forming Units per cubic meter) . . . . . . 97 (2) Adjusted Concentration (CFU/m3) . . . . . . . . . . . . . . . . . . . . . . . . . . 97 (3) Limitations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98 c. Identification of Culturable Bioaerosols . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98 (1) Classical or General Microbiology . . . . . . . . . . . . . . . . . . . . . . . . . . 98 (2) Clinical and Environmental Microbiology . . . . . . . . . . . . . . . . . . . . 99 d. Interpretation of Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

 
 



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